Fig 1: Five cloned HPRT+/− rabbits.
Fig 2: Immunohistochemical staining of (A) IMPDH2 and (B) HPRT in osteosarcoma tissues (Χ400). (C) Distribution of IMPDH2 and HPRT levels in osteosarcoma cohort (N = 127). H&E; hematoxylin and eosin.
Fig 3: Induction of thioguanine resistance and loss of HPRT1 expression in isogenic cells expressing DNA transposase PGBD5. a Stable expression of GFP-PGBD5 and control GFP in BJ-hTERT cells, as assessed by Western blotting against GFP; β-actin serves as loading control. b Clonogenic efficiency of BJ-hTERT cells stably expressing GFP (red) and GFP-PGBD5 (blue) as a function of varying thioguanine concentrations upon thioguanine resistance selection. c Representative photographs of resistant colonies stained with Crystal Violet. d Thioguanine selection of both GFP and GFP-PGBD5 expressing cells yields cells that lack HPRT1 activity, as assessed by hypoxanthine-aminopterin-thymidine (HAT) treatment; *** denotes p = 2.2 × 10−5 and 9.6 × 10−4 for the comparisons between control and thioguanine selected GFP and GFP-PGBD5, respectively. d Thioguanine selection yields thioguanine-resistant cells, as assessed by cellular ATP luminescence assay of GFP (red) and GFP-PGBD5 (blue) expressing cells, as compared to control cells (gray and black). e Western blot for HPRT1 in BJ-hTERT cells expressing GFP and GFP-PGBD5 upon thioguanine selection; β-actin serves as loading control. All error bars represent standard deviations of 3 biological replicates
Fig 4: Antiviral activity of T-705 against rabies virus (RABV) in mouse neuroblastoma cell lines. A, Expression of HPRT in NA and N2a cells. HPRT and β-actin proteins were detected by Western blots. The relative molecular mass is indicated on the left. B, The 1088 or challenge virus standard (CVS) RABV strain was inoculated into NA or N2a cells at a multiplicity of infection (MOI) of 0.01. The cells were incubated for 96 hours in the presence (1000 µM) or absence (0 µM) of T-705. Each virus titer in the supernatant was determined using the focus assay. C, Expression of HPRT in NA cells transfected with the empty or hprt vector. The indicated proteins were detected by Western blots. D, Efficacy of T-705 against NA cells transfected with the hprt vector. The 1088 strain was inoculated into NA cells transfected with the empty or hprt vector or into N2a cells. The cells were incubated for 96 hours in the presence (1000 µM) or absence (0 µM) of T-705. Each virus titer in the supernatant was determined using the focus assay. Data represent the mean ± SD (n = 3). *P < .05, by the Student t test; **P < .05, by 2-way analysis of variance for interaction. Abbreviations: FFU, focus-forming units; NS, not significant.
Fig 5: Overexpressed HPRT1 inhibits dopaminergic neuron loss in 6-OHDA-induced PD mice. 6-OHDA-induced PD mice were treated with oe-NC or oe-HPRT1. (A) Protein expression of HPRT1 in the substantia nigra tissues examined by western blot assay. (B) TH positive neurons in the substantia nigra examined by immunohistochemistry (upper × 100, lower × 400). (C) Fluoro-Jade B-stained apoptotic neurons (scale bar = 50 μm). (D–G) The mRNA expression of Nurr-1 (D), Pitx-3 (E), Ngn-2 (F) and NeuroD1 (G) in the substantia nigra tissues examined by RT-qPCR. *p < 0.05. n = 6. Measurement data are by means ± standard deviation. Comparison between two groups was analyzed by independent sample t test.
Supplier Page from Abcam for Anti-HPRT antibody